Therefore, other data types, such as protein and microRNA expression profiles should be integrated to further reveal these missing actions. At the same time, the network should be updated. Ever-increasing amount of PPIs shall continuously be incorporated into the network. In addition, many other interaction types, such as DNA-protein interaction, transcription factor-target interaction and microRNA-target interaction, shall also be included. It could be envisioned that a comprehensive network with biologically relevant profiles will lead us to more accurate disease molecular signature finding. The interactions between a protein and a drug are of growing interest due to the role in elucidating protein functions, explaining drug action mechanisms and discovering novel drug candidates. G-protein coupled receptors are the largest and most diverse superfamily of integral membrane receptors due to their crucial role in curing varied diseases and being the most important class of drug targets. Currently, a wide range of methods have been developed for investigating the drug-protein interaction, including ultrafiltration, mass spectrometry, X-ray crystallography, nuclear magnetic resonance, surface plasmon resonance, affinity capillary electrophoresis and computer-aided methods. Although contributing greatly to the exploration of drug-protein interactions, these approaches need to be improved to overcome the limitations of long analysis time, requirements for samples with high concentrations or purities, potential interfering substance from the sample and relatively specialized equipment. High performance affinity chromatography is another widely used method for the study of drug-protein interaction. The method often involves immobilization of a protein on a solid matrix to construct affinity stationary phase. Synthesising an assay for attaching a protein to a solid matrix is a key factor for accomplishing an HPAC method. Physical absorption and random immobilised methods are commonly reported ways to synthesis an affinity stationary phase. The types of stationary phases constructed by these methods have been confirmed to generate the inevitable issue of losing bioactive binding sites of the protein during the immobilisation procedure. Oriented immobilisation has proved feasible to address this issue in previous publications and should be further used to synthesise HPAC stationary phases attributed to the particularly important role in life sciences. Beta2-adrenoceptor, one of the members of the GPCRs, is a crucial target of many drugs for fighting obesity and ailments of the heart and respiratory system. Ephedrine and pseudoephedrine are isomers widely used for treating diseases of the respiratory system. Both ephedrine and pseudoephedrine exert their therapeutic action through the same signal pathway involving b2-AR.