Consequently, the transcription of SjM2DH increased significantly with salinity decreasing, which might be due to the function of fructose reduction by M2DH. It is thus presumed that the kelp might keep osmotic pressure through the regulation of the catalytic direction between mannitol and fructose. Naturally, S. japonica niches in sublittoral environments, and has poor acclimation to Linopirdine dihydrochloride desiccation and oxidative stress compared with intertidal algae. Here in this study, remarkable upregulation of SjM2DH was found under desiccation for 1�C2 h and 0.2 to 0.8 mM H2O2 treatment. Long duration of desiccation and higher concentration of H2O2 could not cause a significant transcription of SjM2DH. These results implied that mannitol metabolism might be involved in physiological process response to temperate desiccative and oxidative stress. In addition, a cisregulatory element for drought-response exists in the upstream STOCK2S 26016 sequence of SjM2DH ORF, which could strengthen the function of M2DH under desiccation stress. Although pMAL expression system softened the insoluble expression problems, the detected purified recombinant M2DH exhibited no activity. It may ascribe to the long-term retention at room temperature after cell lysis. Besides, NaCl, EDTA and sodium azide may inhibit the peptide activity. In addition, endogenous M2DH activity was neither detected, which was consistent with extremely lower M2DH activity detected in D. grisea. Considering the characterization of GDP-mannose dehydrogenase from E. siliculosus by constructing a His-tagged MBP recombinant plasmid with a TEV protease cleavage site, more explorations are needed to be further conducted. Renal cell carcinoma arises primarily in the renal parenchyma and accounts for over 90% of kidney carcinomas. Clear cell RCC is the most frequent form of RCC, with an incidence of 75%, followed by non-ccRCC types, including papillary tumors, chromophobe tumors and other rare types. RCC has the highest mortality rate among genitourinary cancers and its incidence has risen steadily, with a global incidence of approximately 200 000 new cases and a mortality rate of more than 100 000 patients annually. Nephrectomy is an effective treatment for localized RCC disease, but advanced RCC is still highly lethal, with a 5-yr survival of 53%. Treatment of RCC is hampered by the limited understanding of the pathogenesis of RCC, particularly the lack of insight into molecular mechanisms and pathways altered during its development.