In order to increase the efficiency of anaerobic digestion of FW, it is necessary to understand the role of the TS contents on the behavior of the Telotristat ethyl microbial community structure involved in the anaerobic digestion of degradation from wet to dry technology. Recently, various molecular microbial ecology tools have been applied in numerous studies to analyze microbial communities in different anaerobic digesters and their influences on the efficiency and stability of AD processes. Pyrosequencing, as a next generation sequencing technology, has gained increasing attention as a novel tool for studying the microbial diversity. Recently, this technology has been widely and successfully used to characterize the microbial community structures in various environmental samples, such as source waster, membrane filtration systems, soil. Meanwhile, the microbial community structures were compared by this technology in anaerobic digestion of food waste at different organic loading rates. Hence, the aim of this study was to conduct a comprehensive comparison of the microbial community structure using 454 high throughput pyrosequencing technology and related these microbial findings to their respective performances of mesophilic anaerobic digesters treating FW with different TS contents ranging from 5%�C20%. It was expected that the reported work herein will reveal the role of the TS content on the behavior of the microbial community structure to increasing TS contents and hence to effective guide high solids anaerobic digestion of FW and to optimize the operational TLR7-agonist-1 conditions for high anaerobic digestion efficiency. Table 2 summarizes the values of the main parameters indicating system stability and potential inhibitory chemicals for three reactors operated at different TS contents, and the performance data were the average values of the last five samples during the operation period of the fifth SRT after the system reached steady state. For each semi-continuously experiment with a good anaerobic digestion performance, there was no accumulation of VFA and low pH. The concentration values of VFA showed increasing trend with increasing TS contents.