Although the breeding CHIC-35 programs for development of high-quality bloodstock have been widely carried out in several countries many shrimp researchers point out that the lack of genetic tools and understanding of molecular mechanisms of Clobenpropit dihydrobromide growth is still a hurdle for effective improvement of growth traits. A better understanding of muscle growth can have significant impacts on overall shrimp growth performances. Skeletal muscle is a remarkably plastic tissue. It can undergo dramatic changes in size and contractile properties during development, as well as when responding to a variety of physiological conditions. Differentiation of skeletal muscles begins when the mesodermal cells in the early embryo become attached to the myogenic lineage, which is then followed by the differentiation of fibers to specific types. In mammals, this involves the expressions of skeletal muscle-specific transcription factors such as MyoD, MFY5, Myogenin, MRF4 and MEF2, which regulate the expressions of muscle-specific genes by interactions with the regulatory DNA sequences of targeted genes. After birth, a diverse number of factors such as hormones, active and passive stretch, use and disuse, and diseases can alter the size and fiber type composition of vertebrate skeletal muscles. Crustacean muscle is structurally analogous to vertebrate skeletal muscles, with proteins organized in sarcomeres aligned along large penniform fibers. The main distinction of crustacean muscle is the different sarcomere length according to fiber type, with fast fibers organized in short sarcomeres and low mitochondrial density, and slow tonic fibers organized in long sarcomeres with high mitochondrial density. In crustaceans, muscle also exhibits a dynamic state of continuous atrophy and restoration to facilitate withdrawal from carapace at molting. The rate of L. vannamei growth rate varies with size, sex and time of year in the coastal waters. Molting frequency varies across different species, but is normally faster in early stages, slows down with age, and is strongly influenced by ecdsyteroid hormones. Muscle loss during molting does not seem to occur in abdominal muscle. Compared with bacterial expression system, insect cell expression system is very useful for functional study of eukaryote protein because it can provide post-translational protein modification.