Conversely, gene expression profile of SLT-treated TM cells after 2 and 6 hours are remarkably different from JK-P3 Control being located in different branches of the dendrogram. A similar situation was recorded by performing Principal Component Analysis of variance where Control and SLT-treated TM cells after 30 min are located in the same quadrant, while SLT-treated TM cells after 2 hours and 6 hours are located in different quadrants far away from Control. Indeed, 15 out of the 67 genes modulated by SLT encoded for activities involved in maintaining TM tissue integrity. Among these, genes involved in adherence to basal membrane and intercellular connection and in extracellular matrix removal resulted up regulated. Conversely, SLT downregulated gene involved in the production of extracellular matrix. These mechanisms contributes to the maintenance of TM Lambrolizumab cellularity counteracting its decrease. It was demonstrated that a relative increase in the extracellular matrix/cells ratio is crucial for POAG progression. IOP homeostasis, triggered by pressure changes or mechanical stretching of the TM, appears to involve the extracellular matrix turnover. An upregulation of genes involved in repairing and removing damaged protein was observed. This finding indicate that SLT increase removal of oxidized proteins typically accumulating in degenerating and aged tissues. Of the 67 genes modulated by SLT 7, encoding for activities involved in cell motility and contraction were upregulated by SLT indicating that this treatment enhance the contracting capacity of TM cells. The contractile function, cell shape, and cell adhesion properties of TM and SC cells have been implicated widely in modulation of aqueous humor outflow through the conventional pathway. Indeed, contraction of TM with its smooth muscle-like properties, decreases outflow, whereas relaxation increases this parameter. This tissue has the ability to contract when exposed to appropriate stimulants, its property is important in helping regulate the outflow of aqueous humor. Indeed, Trabecular meshwork cells express contractile elements such as smooth muscle a-actin and myosin.