Currently half of the infections are acquired in The Netherlands where the serovars Copenhageni, Icterohaemor-rhagiae and Grippotyphosa are dominant. This might induce a bias of the performance of the test. For this reason,high content screening the last stages of the OIE validation scheme include field studies at other laboratories to assess clinical sensitivity and specificity under different circumstances. We are currently aiming at the imple-mentation and evaluation of the test in endemic areas with a variety of causative serovars. In this study, culture and serology were considered as gold standard to estimate the clinical sensitivity and specificity in order to measure eventual bias of the results of high bacterial loads in culture and PCR positive samples alone. The overall sensitivity and specificity in this study were estimated as 93% and 100%, respectively. The assay showed complete reproducibility and repeatability as well as high level of robustness since changing in critical PCR parameters has no or slight influence on overall results. Testing kidney, lung and liver from two early deceased patients as well as some rodent kidneys proved clearly the usefulness of the real-time PCR as an effective tool for the detection of Leptospira in the distinct tissues. This shows the applicability of real-time PCR as a suitable diagnostic tool on post-mortem samples, Life Science Reagents overcoming the failure to confirm leptospirosis of early deceased patients by serology. The PIM2 kinase belongs to a family of three serine/threonine kinases first identified as preferential proviral insertion sites in Moloney Murine Leukemia Virus induced T-cell lymphomas. In humans PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas. Importantly, expression of the pim2 transgene predisposes mice to T-cell lymphomas and is highly cooperative with the Em-myc transgene in the development of pre-B cell leukaemia. Located on the X chromosome the pim2 gene is highly induced by growth factors and cytokines through STAT5 activation.