Chemical IKK-16 chromatographic fingerprinting by high performance liquid chromatography and ultraperformance liquid chromatography coupled with electros pray ionization and quadrupole time of flight mass spectrometry, in combination with principal component analysis have been proven to be a powerful platform for metabolomics studies, as they not only are useful for comparing the chemical profiles of herbal medicines, but also for identifying chemical markers or indices. In this study, we intentionally Levodropropizine combined all of these various technology systems mentioned above for and integrated investigation for phytomedicine use of a specific medicinal herb, that we recently demonstrate to confer strong anti-colitis activity in experimental mouse system. Inflammatory bowel disease, with a high incidence worldwide, is a result of chronically relapsing idiopathic inflammation of the gastro intestinal tract. Patients with IBD typically also run a high risk of the disease developing into colorectal cancer due to chronic damage and inflammation in the colon and tissues of the rectum. The DSS-induced mouse colitis model is well accepted for studying IBD due to its resemblance to human ulcerative colitis. Drugs for treating IBD including 5-aminosalicyclicacid, sulfasalazine, and others; however, IBD patients usually need long-term and repeated treatment, involving potentially toxic drug doses. Because of such side-effects or/and lack of effectiveness of standard western medicine therapies, many IBD patients turn to complementary and alternative medicine. Herbal therapy is the most common type of CAM used for gastrointestinal disorders, but so far there have been limited reports of efficacious CAM therapeutic strategies or safe phytomedicines to remedy IBD. Wedelia chinensis belonging to family Asteraceae is commonly consumed orally. It is often infused with hot water to make an herbal tea. Such herbal tea drinks, as cold beverages, are readily available commercially other Asian countries. We revealed in our previous study that the crude plant extract of W.chinensis can confer a specific anti-inflammatory effect on dex transulphate sodium-induced murine colitis.