Our results on the potential toxicity of zidovudine during pregnancy cannot be directly compared with other studies because of the different durations of zidovudine exposure, haematological parameters considered, and overall study designs. Finally, the conditions used for HT protein expression were by necessity optimized for a wide range of proteins. The use of individualized conditions might result in improved results for particular proteins. The protein expression clones generated in this study can readily be used to scale up protein expression to achieve higher amounts of protein if required. The use of the Gateway recombinational cloning system to build the clones further facilitates easy shuttling of ORFs into a variety of protein expression vectors with different affinity tags. As this collection includes a normalized stop codon at the end of the ORF, this collection is restricted to adding tags at only the N-terminus. Automated protein analysis obviated the need to use gel-based chromatography and resulted in a quantitative digital output of protein concentration and purity. This form of output allowed normalization of protein concentration prior to use in Gypenoside-XVII downstream assays. Because our past experience suggested that HT experimentation could be error prone, we took precautions at every step to minimize manual intervention and ensure efficient tracking at the plate and sample levels. All adult multicellular organisms with either simple or complex body plans harbor Neferine somatic stem cells. These are undifferentiated cells found throughout the body that are able to renew themselves through mitotic processes, and have the capacity to generate daughter cells able to differentiate into a variety of specialized cells. In mammals, adult stem cells occur in numerous organs and tissues and are characterized by a remarkable level of plasticity. The stimulation to differentiate into a specific cell type for somatic stem cells appears to largely be a series of internal and external signals, which can include chemical and molecular signals such as cytokine and growth factor secretion within a predetermined microenvironment that surrounds the stem cell and provides determinant clues The plasticity of somatic stem cells has stimulated great interest due to their numerous potential applications, in addition to basic and in clinical research, as a substitutive or regenerative therapy for numerous human diseases or injuries.