Both DLG4 and KCNMA1 are key regulation proteins of mitochondrial enzyme complexes involved in the cellular response to oxidative stress, a process that is also described in EAE/MS. We were able to detect DLG4 and KCNMA1 in our samples, but they were not differential between the experimental groups. This could indicate that the activation of these molecules does not influence their expression. The alterations in their downstream molecules however suggest that these pathways are activated during the different disease stages and thus involved in disease mechanisms. AR/ PSA, and Akt signaling, which makes them very promising agents for the prevention and/or treatment of PCa and its bone metastasis in combination with conventional chemotherapy. MS is an inflammatory autoimmune disease of the central nervous system in which genetic, environmental and immunological factors are involved. The disease is characterized by blood brain barrier breakdown, demyelination, oligodendrocyte apoptosis, progressive axonal damage and reactive astrogliosis. These pathological hallmarks are present in the multifocal inflammatory lesions of the CNS, primarily localized in the white matter.Indeed,3-Methylsalicylic acid DLG4 immunoreactivity in both gray and white matter of EAE spinal cord tissue was reciprocally associated with damage of postsynaptic structures and directly associated with disease activity. When EAE animals were in remission, DLG4 expression partly restored, further emphasizing the actual involvement of the DLG4 pathway in disease. Furthermore, we showed that specific blocking of KCNMA1 in macrophages decreased the ability of macrophages to phagocytose myelin, a pathological hallmark of MS and EAE lesions. The infiltration of autoreactive T cells, B cells and macrophages, and the production of pro-inflammatory cytokines are known to take part in the formation of inflammatory CNS lesions. Still, the exact cause and underlying molecular mechanisms remain poorly understood, but are crucial in the search for new therapeutic options. A proteomics approach was chosen to get more insight in the molecular processes of MS. Proteomics studies are valuable to get an overview of protein expression in cells,(R)-Oxiracetam tissues or organisms. These protein expression profiles can provide indications towards molecular mechanisms involved in normal and disease processes. In the past, gel-based proteome studies of brain and cerebrospinal fluid were carried out by comparison of intensities of stained gel spots, a procedure that may suffer from experimental variability and poor reproducibility. Only adequate quantitative approaches will allow the analysis of disease processes over time in the brain or CSF during neuroinflammation. Two-dimensional fluorescence difference gel electrophoresis is a very sensitive gel-based proteomics technique that is unique through the utilization of fluorescently labelled samples on the same gel, and the application of an internal standard for intra- and inter-gel comparisons and normalization.