These markers have been especially useful in identification of male germ cells while isolation and purification from the testis. Identification of a Publications Using Abomle GSK1120212 species-specific marker can aid in tracing spermatogonia of the donor species in xenotransplanted mice testis. Recently, we reported lectin DBA as a specific marker for spermatogonia in testes of prepubertal buffalo, and utilized it for their characterization in short-term culture. However, stem cell potential of Cathepsin inhibitor 1 Abmole Hispidin induces autophagic and necrotic death in SGC-7901 gastric cancer cells through lysosomal membrane permeabilization by inhibiting tubulin polymerization buffalo spermatogonia has not yet been determined. The objective of the present study was to examine the expression of POU5F1 transcript and protein in prepubertal and adult buffalo testes. Further, stem cell potential of buffalo gonocytes/spermatogonia, isolated from prepubertal testis, was determined using the testis xenotransplantation assay. To determine the stem cell potential of gonocytes/spermatogonia, testicular cells from prepubertal buffalo testes were transplanted into the testes of immunodeficient mice. The injected spermatogonia migrated to the basement membrane of the seminiferous tubule and appeared as chain of cells that were stained with DBA. These cells were located in the area of the seminiferous tubule, consistent with the stem cell niche. Occasionally, a few DBA-positive cells were seen in the lumen of seminiferous tubules. DBA-positive cells were not found in the contralateral testis, used as control. Pou5f1 is a transcription factor required to maintain the pluripotency and self-renewal of ES cells. Pou5fl controls a cascade of pathways that are intricately connected to govern pluripotency, self-renewal, genome surveillance and cell fate determination. To investigate the stem cell characteristics of germ cells in buffalo, we examined the expression of Pou5f1, both at transcript and protein level in prepubertal and adult testes. In the present study, expression of POU5F1 transcript was detected both in prepubertal and adult buffalo testis. In a recent study, expression of POU5F1 transcript was reported in buffalo embryonic stem cell like cells. However in the same study, it was reported that POU5F1 is expressed as four pseudogenes. The POU5F1 primers used in this study amplified a PCR product of a definite size in buffalo testes. It is likely that the primers designed in the present study were in a conserved region of buffalo POU5F1 gene. The possibility of existence of a single transcript for the POU5F1 gene in buffalo testis cannot be ruled out. Western blot analysis showed that the anti-POU5F1 antibody identifies two isoforms of POU5F1 protein buffalo testes. The smaller fragment in POU5F1 immunoblots indicates towards the cytoplasmic isoform of POU5F1 protein in buffalo testis.