Their vertebrate hosts, and form quadrinucleated, chitinaceous cysts with osmotically resistant cyst walls. E. invadens will readily encyst in vitro in response to carbon source deprivation, hypoosmotic shock, or a combination of the two stimuli. As most of current drugs against protozoa target metabolism, it is critical to understand the structure and dynamics of the parasite metabolic network during encystation. Indirect approaches to reconstructing the metabolic network, by comparative genomics and enzymological studies of individual enzymes, are at the best incomplete and face major obstacles in highly divergent organisms such as parasitic protozoa. Global metabolomics is a new and powerful technology that provides a relatively complete picture of the metabolism in biological systems and has recently been applied to a wide variety of important problems. We decided to apply this approach to understand the basis of the changes in cellular metabolism that occur during encystation. To better understand the relationship between gene expression and metabolites levels, we also analyzed the mRNA expression profile of the enzymes involved in the formation or utilization of these metabolites. Current evidence suggests that inherited risks play a role in XAV939 glioma susceptibility, as with other cancers. A majority of the inherited risk is due to the co-inheritance of multiple low-risk variants, some of which are commonly seen gene variants and hence can be identified through association studies. The epidemiology of glioma has focused on identifying factors that can be modified to prevent this disease. Recent research has focused on identifying germ line polymorphisms associated with the risk of glioma and defining molecular markers to classify glial tumors in more homogenous groups. The epidermal growth factor receptor regulates important cellular processes and is implicated in human tumors. Several previous studies have assessed single nucleotide polymorphisms in the EGFR gene for the association with the risk of cancers, such as lung cancer, breast cancer, prostate cancer, and esophageal cancer. Previous studies have shown that regulation of the EGFR pathway plays an important role in glioma progression, and certain EGFR genotypes may be related to glioblastoma risk, indicating that germline EGFR polymorphisms may have important implications in carcinogenesis of glioma. In addition, it is possible that haplotypes and locus–locus interactions within the EGFR gene may be correlated with the development of glioma. To investigate potential relationships between EGFR SNP polymorphisms, haplotypes, locus–locus interactions, and their role in the etiology of gliomas, we performed a comprehensive association analysis in a case–control study in the Han Chinese population. Our study indicated important evidence for the association between EGFR gene polymorphisms and the risk of glioma. In this case–control study in a Han Chinese population, we identified for the first time rs1468727 and rs730437 in the EGFR gene associated with an increased risk of glioma.